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Protein persulfidation is one of the most important oxidative translational modifications and plays vital roles in various important biological processes. However, the proteome-wide identification of persulfidation sites is a great challenge because of the difficulties in accurately differentiating persulfide groups with disulfide and thiol groups in proteins as well as the extremely low abundance of persulfidated peptides.
By current approaches, the persulfidated peptides were often identified by the cleavage of their persulfide groups by reductants prior to MS analysis; therefore, it would bring about a false positive identification and was unable to identify persulfidation sites accurately for a single peptide with multiple cysteine residues. In this study, a novel strategy for the site-specific quantification of persulfidome SSQPer was developed. By this strategy, the persulfidated proteins were first labeled with cleavable isotope-coded affinity tag c-ICAT reagents.
To evaluate the performance of SSQPer, the persulfidated BSA digests with 20 persulfidation sites identified were used to spike HeLa cell digests with mass ratios of and , and 16 and 13 persulfidated sites were respectively identified.
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Public Health Insurance Marketplaces are available in every state in the U. Also known as public exchanges, these online shopping hubs are available for individual consumers and small businesses to compare plans and buy health insurance. Since their introduction in , public Marketplaces have faced a variety of challenges, from enrollment and plan participation particularly among younger adults , to costs and delays. In most states, the Open Enrollment period runs from November 1, through December 15 with coverage effective on January 1.
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Table of Contents. Registration No. Exact name of registrant as specified in its charter. Gregg J. Approximate date of commencement of proposed sale to the public: As soon as practicable after this Registration Statement becomes effective. Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting company. Check one :. The information in this preliminary prospectus is not complete and may be changed.
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